Gpx4 Stable Expression Cell Line (RAW 264.7)

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  • 细胞图0 Gpx4 Stable Expression Cell Line (RAW 264.7)-细胞图0
  • 细胞图1 Gpx4 Stable Expression Cell Line (RAW 264.7)-细胞图1
  • 质粒图0 Gpx4 Stable Expression Cell Line (RAW 264.7)-质粒图0
  • Gpx4 Stable Expression Cell Line (RAW 264.7) Gpx4 Stable Expression Cell Line (RAW 264.7)-产品图
细胞图0 Gpx4 Stable Expression Cell Line (RAW 264.7)-细胞图0
细胞图1 Gpx4 Stable Expression Cell Line (RAW 264.7)-细胞图1
质粒图0 Gpx4 Stable Expression Cell Line (RAW 264.7)-质粒图0
Gpx4 Stable Expression Cell Line (RAW 264.7) Gpx4 Stable Expression Cell Line (RAW 264.7)-产品图
Gpx4 Stable Expression Cell Line (RAW 264.7) Copy Product Info
Catalog#： YOE-L029
Size： 1*10^6
Culture method： 90%DMEM+10%FBS
STR Report
Datasheet (Use Instruction)
  • 细胞图0 Gpx4 Stable Expression Cell Line (RAW 264.7)-细胞图0
  • 细胞图1 Gpx4 Stable Expression Cell Line (RAW 264.7)-细胞图1
  • 质粒图0 Gpx4 Stable Expression Cell Line (RAW 264.7)-质粒图0
  • Gpx4 Stable Expression Cell Line (RAW 264.7) Gpx4 Stable Expression Cell Line (RAW 264.7)-产品图
Gpx4 Stable Expression Cell Line (RAW 264.7) Copy Product Info
Catalog#： YOE-L029
Size： 1*10^6
Culture method： 90%DMEM+10%FBS
Datasheet (Use Instruction)

Detailed Product Information

Cell Info

Catalog YOE-L029 Transcript NM_001037741.4
Cell name Gpx4 Stable Expression Cell Line (RAW 264.7) Species Mouse
Gene Name Gpx4 Passage ratio 1:2-1:3
Cell Morphology monocyte/macrophage,adherent Antibiotic Concentration Puro=3μg/ml
Culture method 90%DMEM+10%FBS Mycoplasma test negative
Catalog
YOE-L029
Cell name
Gpx4 Stable Expression Cell Line (RAW 264.7)
Gene Name
Gpx4
Cell Morphology
monocyte/macrophage,adherent
Passage ratio
1:2-1:3
Transcript
NM_001037741.4
Species
Mouse
Antibiotic Concentration
Puro=3μg/ml
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS

Product Description

The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Disruption of this gene in mouse spermatocytes is associated with male infertility. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Transcript variants resulting from alternative splicing or use of alternate promoters have been described to encode isoforms with different subcellular localization. Pseudogenes of this locus have been identified on chromosomes 10 and 17.
Gene Expression Validation
No. Sample mGpx4-1CтMean Q-mGAPDHCтMean ΔCтMean ΔΔCт Expression Rate
1 Gpx4 Stable Expression Cell Line (RAW 264.7) 23.977 13.356 10.621 -6.410 85.036
2 RAW264.7_Ctrl(Puro) 30.749 13.718 17.031 0.000 1.000
STR Profile
Download STR Report
Loci STR Info (Sample Cell)
Sample Cell Line:RAW 264.7		 STR Info (Cell bank)
Cell Line:RAW 264.7
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
18-3 18.018.0
4-2 22.322.3
6-7 12.012.0
19-2
1-2 17.0
7-1 25.225.2
8-1 13.013.0
1-1 15.016.0
3-2 14.014.0
2-1 16.016.0
15-3 22.322.3
6-4 18.018.0
13-1 16.216.2
11-2 17.017.0
17-2 14.016.014.016.0
12-1 16.016.0
5-5 14.014.0
X-1 24.024.0
TH01
D5S818
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • icon
    High expression
    Exclusive cell selection technique, ensuing high expression, RT-qPCR validation data provided.
  • icon
    Good stability
    Stable gene copy number, low passage, can be stably passaged for more than 20 passages.
  • icon
    Strict QC
    Strict quality control, ensuing no bacterial and mycoplasma contamination, STR authentication and cell post-thawing viability test passed; High standard delivery.
Related Resources: Red Cotton Gene-editing System | ATCC | DSMZ | ECAZZ | Cellosaurus | CCTCC | NCBI
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